The Use of Molecular Markers in Searching for Tomato Fusarium Blight Resistance Genes
I. N. Shamshin1, Yu. V. Gryazneva2, M. V. Maslova3
1N. Shamshin, Michurinsk State Agrarian University, Michurinsk, Russia.
2Yu. V. Gryazneva, Michurinsk State Agrarian University, Michurinsk, Russia.
3M. V. Maslova, Michurinsk State Agrarian University, Michurinsk, Russia.
Manuscript received on 23 March 2019 | Revised Manuscript received on 30 March 2019 | Manuscript published on 30 March 2019 | PP: 1800-1803 | Volume-7 Issue-6, March 2019 | Retrieval Number: F2807037619/19©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: The paper presents the result of screening a collection of varieties and forms of tomatoes from the Michurinsk State Agricultural University with the use of molecular markers of Fusarium blight resistance genes. 62 tomato genotypes have been tested using molecular markers I-2 and At-2, which allow identifying the genes of resistance to the first and the second race of Fusarium blight pathogen — Fusarium oxysporum. Marker I-2 is codominant. It has been used to identify six genotypes with gene I-2 in the heterozygous state. Gene I-1 has been identified using the dominant marker At-2. As a result of the molecular-genetic analysis, 19 genotypes with the dominant homozygous allele of the sought gene have been identified. The identified genotypes may be used as donors and sources of resistance genes for further breeding work.
Keywords: Tomato, Breeding, Resistance genes, Fusarium Oxysporum, Molecular markers, PCR.
Scope of the Article: Soil-Structure Interaction