Quantitative Estimation of Gallic Acid and Rutin of Non-Gilled mushrooms Collected from North-Western Himalayas by HPTLC
Shaveta Singh1, Isha Sharma2, Astha Tripathi3
1Dr. Shaveta Singh, Faculty of Appplied Sciences and Biotechnology, Shoolini University, Bajhol, Solan (HP), India.
2Isha Sharma, Faculty of Appplied Sciences and Biotechnology, Shoolini University, Bajhol, Solan (HP), India.
3Dr. Astha Tripathi*, Assistant professor, Faculty of Appplied Sciences and Biotechnology, Shoolini University, Bajhol, Solan (HP), India.
Manuscript received on January 02, 2020. | Revised Manuscript received on January 15, 2020. | Manuscript published on January 30, 2020. | PP: 372-379 | Volume-8 Issue-5, January 2020. | Retrieval Number: D9793118419/2020©BEIESP | DOI: 10.35940/ijrte.D9793.018520
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: To carry out the quantitative estimation of Gallic acid and Rutin from the methanolic extract of four non-gilled mushrooms by high-performance thin layer chromatography (HPTLC) method. Material and method: Four non-gilled mushrooms (Auricularia auricula-judae, Auriuclaria polytricha, Ganoderma lucidum and Trametes elegans) were collected from North Western Himalayas during the rainy season. Stationary phase for this study was TLC aluminium sheets precoated with silica gel 60 F254 and mobile phase was Toluene: Ethyl acetate: Formic acid (3:6:1v/v) and Chloroform: Ethyl acetate: Methanol: Formic acid (3.5:5:0.5:1 v/v) for Gallic acid and Rutin respectively. The detection and quantification of Gallic acid and Rutin were performed at 280 nm and 254 nm respectively. Results: Rf value of Gallic acid and Rutin were recorded to be (0.43) and (0.70) respectively. Both HPTLC peak areas for standards Gallic acid (6467.8) and Rutin (16592.4) were compared with corresponding peak areas of four non-gilled mushroom extract and the amount of Gallic acid was calculated. All the four non-gilled mushrooms extracts showed the good concentration of Gallic acid and Rutin. But the amount of Gallic acid was higher in comparison to Rutin. Conclusion: This study results showed that the four non-gilled mushrooms extracts are potential sources of antioxidant activity.
Keywords: Gallic acid, HPTLC, Non-gilled mushrooms, Rutin.
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