Characterization of Lectin from Colpomenia Sinuosa and Effect of Physico Chemical Parameters on Haemagglutination Activity
Malini. M. M1, Jansi. M2, Sini Margret. M3, Anooj. E. S4, Lekshmi Gangadhar5

1Malini M. M, Research Scholar, Department of Zoology, M. S. University, Tirunelveli, Research S. T. Hindu College, Nagercoil (Tamil Nadu), India.
2Jansi M, Associate Professor, Research Department of Zoology, S. T. Hindu College, Nagercoil (Tamil Nadu), India.
3Sini Margret. M, Assistant Professor, Department of Zoology, Nanjil Catholic College of Arts and Science, Kaliyakkavilai, Kanyakumari (Tamil Nadu), India.
4Anooj E. S, Director, Xcellogen Biotech India Pvt Ltd, Nagercoil (Tamil Nadu), India.
5Lekshmi Gangadhar, Nano Dot Research Pvt Ltd, Nagercoil (Tamil Nadu), India.
Manuscript received on 26 November 2019 | Revised Manuscript received on 04 December 2019 | Manuscript Published on 10 December 2019 | PP: 844-848 | Volume-8 Issue-3S2 October 2019 | Retrieval Number: C12561083S219/2019©BEIESP | DOI: 10.35940/ijrte.C1256.1083S219
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Abstract: Lectin is a protein which has the ability to bind carbohydrates and named as haemagglutinin. Lectins with specific carbohydrate specificity have been purified from various plant tissues and other organisms and exploited extensively in many aspects of biochemistry and biomedicine. Similar to land plants, lectins from marine algae appear to be useful in some biological applications. Although several studies on lectins from marine algae have been reported till date, few lectins from algae have been characterized in detail. The present study was focused on the lectin isolated from C.sinuosa. The algal lectin has high sugar specificity with N-acetylglucosamine and higher enzyme activity with trypsin. This lectin was identified as CaCl2 dependent – ‘C’ type lectin and was sensitive to EDTA. Higher H.A titre value was observed with CaCl2 and the lower with MnCl2 and ZnCl2 . Significant lectin activity was observed between pH 7 to 8 and temperature between 20 to 40 O C.
Keywords: Lectin, Algae, Haemagglutinin, Biochemistry.
Scope of the Article: Computational Biology