Screening of Phytochemical Compounds and Assessment of Antiurolithiatic Activity of Pisonia Alba Leaves Extracts against DNA Damage
Kavitha Rani Mari1, Suriyavathana Muthukrishnan2, Punithavathi Manogaran3, Anandhi Eswaran4
1Kavitha Rani Mari, Department of Biochemistry, Periyar University, Salem (Tamil Nadu), India.
2Punithavathi Manogaran, Department of Biochemistry, Periyar University, Salem (Tamil Nadu), India.
3Anandhi Eswaran, Ph.D Research Scholars, Department of Biochemistry, Periyar University, Salem (Tamil Nadu), India.
4Dr. Suriyavathana Mthukrishnan, Associate Professor, Department of Biochemistry, Periyar University, Salem (Tamil Nadu), India.
Manuscript received on 19 September 2019 | Revised Manuscript received on 06 October 2019 | Manuscript Published on 11 October 2019 | PP: 432-435 | Volume-8 Issue-2S10 September 2019 | Retrieval Number: B11020982S1019/2019©BEIESP | DOI: 10.35940/ijrte.B1102.0982S1019
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: This work investigate the Phytochemical constituents of Pisoniaalba (PA) leaves using standard protocols and to determine its DNA damage inhibition and Antiurolithiatic capability. Dried leaves of Pisoniaalba were used to screen and quantify the phyto constituents in different organic solvents extracts that are analysed by GCMS, FTIR and HPTLC. In the presence of PUC18 plasmid DNA, the DNA damage inhibition assay was performed by photolysing H2O2 with UV radiation and agarose gel electrophoresis with irradiated DNA. The invivo antiurolithic assay was performed by nucleation method. Pisoniaalbain’s phytochemical analysis reveals the existence of secondary metabolite components including glycosides, resins, phenols, terpenoids, flavonoids, tannins, steroids, and alkaloids, etc.. ELPA express the maximum quantity of phenols, flavonoids, alkaloids and terpenoids which were 30.44 ±0.65 mg TAE/g extract, 28.51 ± 1.19 mg RE/g extract, 28.08 ± 0.08 mg of AE/g of extract, 29.94 ± 0.32 mg RE/g extract respectively. The GC-MS assessment findings confirmed the presence of nine ELPA phyto compounds accompanied by eleven ALPA phytoc ompounds. It was also observed that Pisoniaalba leaves prevents DNA damage in UV and H2O2 treated plasmid DNA. The invitro Antiurolithiatic research indicates that ELPA was more efficient in blocking calcium oxalate nucleation. From the obtained results it was proven that the Pisonia alba leaves has potential secondary metabolites with therapeutic role mainly against urolithiasis. Both invitro and in vivo analysis demonstrates that ELPA significantly reduces crystal formation that are causative of renal stones. These evidences suggest that Pisoniaalbacan be further investigated for the prevention and treatment of urolithiais.
Keywords: ALPA, DNA fragmentation, ELPA, Urolithiasis, Pisoniaalba, Nucleation.
Scope of the Article: Bio-Science and Bio-Technology