Effect Type of Solvent for Extraction Binahong Leaves (Anrederacordifolia (Ten.) Steenis) on Saponin Levels by Gravimetric Method
Sri Wardatun1, Sutanto2, Euis Mahmudah3
1Sri Wardatun, Department of Pharmacy, Pakuan University, Indonesia.
2Sutanto, Department of Chemistry, Pakuan University, Indonesia.
3Euis Mahmudah, Department of Pharmacy, Pakuan University, Indonesia.
Manuscript received on 04 August 2019 | Revised Manuscript received on 27 August 2019 | Manuscript Published on 05 September 2019 | PP: 298-299 | Volume-8 Issue-2S7 July 2019 | Retrieval Number: B10230782S719/2019©BEIESP | DOI: 10.35940/ijrte.B1023.0782S719
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Secondary metabolites, also known as phytochemicals, natural products or plant constituents are responsible for medicinal properties of plants. The Secondary metabolite compounds contained in binahong leaves were steroids, alkaloids, flavonoids, saponins, polyphenols and tannins. The purpose of this research was for determine the content of saponin compounds in binahong leaves extract by gravimetric method with differents type of solvent. Binahong leaves made into simplicia and extracted using 3 types of solvents: 70% ethanol, 95% ethanol and methanol. The extraction was performed in reflux. The extract fractionated with n-hexane, ethyl acetate and n-butanol solvent, respectively. The levels of saponin in fraction n-butanol determined by gravimetric methods. The results showed that the type of solvent had an effect on the levels of isolated saponins. The methanol solvent produced the highest saponins in 54.30%, 95% ethanol in 35.84% and 70% ethanol in 25.91%, respectively.
Keywords: Binahong Leaves, Saponins, Methanol, 70% Ethanol, 95% Ethanol.
Scope of the Article: Bio-Science and Bio-Technology